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Anti-Müllerian Hormone (AMH)

產(chǎn)品參數(shù)

Anti-AMH Antibody (Datasheet)

Source Mouse monoclonal antibody, cultured in vitro
Catalog number K27n8 K25h6 K59a5 K60b6
Applications Detection Capture Capture Detection
Platforms Immunofluorescence Chemiluminescence
Buffer 1x PBS
Purity >98%,purified by Protein A/G chromatography
Storage instructions Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Product Information

Antibody Product Information

Antibody Evaluation (Immunofluorescence-based)
Immunofluorescence-Based test strip was prepared with K27n8-K25h6 matched antibody pairs (30 Beckman calibrators, Concentration: 0.5-30 ng/ml, R2>98%)
免疫熒光平臺(tái)樣本符合率

Fig. 1 Coincidence rate of Immunofluorescence-based sample

Accuracy
The quality control samples with concentration of 1.5 ng/ml-2.5 ng/ml and 15 ng/ml-25 ng/ml respectively were required to repeat test three times. B= (M-T) / T×100% (B: Relative Standard Deviation, M: Average, T: Concentration)
Sample
(ng/ml)
Test 1 Test 2 Test 3 Average
(ng/ml)
RSD Standard
Batch 1 2.18 2.29 2.31 2.41 2.34 7.19% ±15%
21.29 23.15 22.51 23.97 23.21 9.02%
Batch 2 2.18 2.35 2.13 2.29 2.26 3.52%
21.29 19.4 24.78 22.65 22.28 4.63%
Batch 3 2.18 2.39 2.38 2.34 2.37 8.72%
21.29 23.24 20.55 22.72 22.17 4.13%
Repeatability
The quality control samples with concentration of 1.5 ng/ml-2.5 ng/ml and 15 ng/ml-25 ng/ml respectively were required to repeat test over 10 times. CV= (S /average) ×100% (CV < 15% as indicated in the following table)
Low Value
(2.18ng/ml)
High Value
(21.29ng/ml)
Test 1 2.09 25.36
Test 2 2.06 24.17
Test 3 2.55 23.65
Test 4 2.52 25.17
Test 5 2.11 25.18
Test 6 2.33 21.36
Test 7 2.41 20.91
Test 8 2.39 26.55
Test 9 2.05 21.19
Test 10 2.47 23.87
Average(ng/ml) 2.30 23.74
SD 0.20 1.97
CV 8.72% 8.31%
Standard ±15%
Clinical Significance

Description

Anti-Müllerian hormone (AMH) is a 140 kDa disulphide-linked homodimeric glycoprotein and a member of the transforming growth factor-β (TGF-β) superfamily of growth and differentiation factors. In the ovary, AMH has an inhibitory effect on primordial follicle recruitment and growth. It is also responsible for testicular development.
AMH tests may be used to evaluate ovarian reserve, monitor women who have certain types of ovarian cancer, help diagnose polycystic ovary syndrome (PCOS) and predict the start of menopause. AMH is the most sensitive component and the most accurate existing refection of ovarian reserve among the biomarkers. The ovarian reserve testing allows for tailored fertility treatment plans.
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